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Hi forum,
I am analyzing counts data from Tophat bam followed by HTSeq.
I have 11 biological replicates for group 1 and 10 biological replicates for group 2.
Looking at the attached dispersion plots, is it a fair interpretation that there
is too much within group dispersion (most values around 1) and within group dispersion is simply too large for anything called differentially expressed? I found ~150 genes with p<0.05 and none of the survived 0.1 FDR (giving padj=1 for all).
What'd would be a suggested way to analyze this data further?
Regards,
WDT
I am analyzing counts data from Tophat bam followed by HTSeq.
I have 11 biological replicates for group 1 and 10 biological replicates for group 2.
Looking at the attached dispersion plots, is it a fair interpretation that there
is too much within group dispersion (most values around 1) and within group dispersion is simply too large for anything called differentially expressed? I found ~150 genes with p<0.05 and none of the survived 0.1 FDR (giving padj=1 for all).
What'd would be a suggested way to analyze this data further?
Regards,
WDT