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m_elena_bioinfo 11-11-2009 08:45 AM

bfast postprocess error
Dear users,
I'm running a file fastq of microRNA reads with bfast, using as reference the microRNA precursor sequence.

The step "localalign" runs only if I set the parameter -u (Do ungapped local alignment).

I run the bfast postprocess step as:
>bfast postprocess -f hsa_human_miRNAprec_miRBaseV13.fasta -i sample_622T_9.baf -a 3 -O 3 -o sample

but it returns me an error and it crashes:

In function "GetReverseComplimentAnyCaseBase": Fatal Error[OutOfRange]. Message: Could not understand sequence base.

Has someone had the same problems?
Thanks very much!
Bye bye,
Maria Elena

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