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-   -   Miseq custom primer design, primer linker (http://seqanswers.com/forums/showthread.php?t=57843)

Cyrus Taheri 04-21-2015 01:34 PM

Miseq custom primer design, primer linker
 
Miseq custom primer design, primer linker

Many papers have used a 2bp primer linker in 5 end of the amplicon primers. As, I understand this is to prevent taxon specific PCR bias due to matches to the overhanger and/ or barcodes. I am wondering why 16S protocol did not add a linker.
Thanks,

nucacidhunter 04-21-2015 05:25 PM

Good question for Illumina. Addition of linker does make sense with no known or presumed detrimental effect.


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