Hi,
I am working with RRBS methylation data. At the moment I have got the sequencing done and have the FAST QC results of all the sequenced samples.
There was one sample during bisulphite conversion process that showed white crystalline precipitate after the incubation of 18 hours after adding bisulphite conversion reagent. I am assuming that might have happened due to incomplete bisulphite conversion of the sample.
Is there any way to check through FASTQC, if the sample is partially bisulphite converted?
Thanks for your help.
I am working with RRBS methylation data. At the moment I have got the sequencing done and have the FAST QC results of all the sequenced samples.
There was one sample during bisulphite conversion process that showed white crystalline precipitate after the incubation of 18 hours after adding bisulphite conversion reagent. I am assuming that might have happened due to incomplete bisulphite conversion of the sample.
Is there any way to check through FASTQC, if the sample is partially bisulphite converted?
Thanks for your help.
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