What methods are normally used to trim off the unreliable poor-scoring ends of reads? Is there a tool that can statistically assess read scores and make this decision on a per-read basis, or is a cut position globally decided on?
Are there any special considerations for paired-end reads? (they've already been split and de-convoluted by barcode by the pipeline in our university's sequencing core.)
Are there any special considerations for paired-end reads? (they've already been split and de-convoluted by barcode by the pipeline in our university's sequencing core.)
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