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-   -   Bioanalyzer expired RNA Nano 6000 kit (http://seqanswers.com/forums/showthread.php?t=23666)

arkal 09-27-2012 05:11 AM

Bioanalyzer expired RNA Nano 6000 kit
 
Hey!

I'm need to run a preliminary QC on my RNA samples but I've got a problem... My RNA Nano 6000 kit expired in june 2010 (yes yes lmao :D ).

I wanted to know whether only reagents have an expiry date or does the chip also have an expiry date? I have 90+ unused chips all from the same lot (expiring June 2010) and dont know whether i can run my assay on the same chips if i buy fresh reagents and ladder or whether i need to buy new chips as well. Any help will be appreciated!

thanks in advance
Arkal

cascoamarillo 09-27-2012 08:30 AM

Hi,
I've used expired RNA pico reagents and chips (>1 year) without to much trouble (from different lots); it gave me really good profiles. Maybe, the ladder is the thing that shouldn't be too old. I think your chips from 2010 are gonna be fine with fresh reagents. Let me know how it works if you do so.
Cheers

pbluescript 09-28-2012 06:45 AM

I just wanted to second cascoamarillo's comments. I've never had a problem with old chips. The dye and ladder are the reagents that have given me trouble if they are old.

arunkh 10-08-2012 11:36 PM

Hi,

You shouldnt be having any problem with the Chips whatsoever. But the reagent should be gone. The reagents have to be reordered. RNA 6000 reagents were a lot of trouble to us even when it hadn't expired.


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