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-   -   splitting Tophat2 bam files in separate samples (http://seqanswers.com/forums/showthread.php?t=101946)

Dixi06 08-13-2021 07:03 PM

splitting Tophat2 bam files in separate samples
 
Hello everyone,

I have RNA seq data of 12 samples (paired end, so 24 files) and I ran tophat2 for alignment on all of them which gave me one bam file for all the samples. Now I need separate bam files for all the samples. Can I spilt them from the one I have or I have to do tophat2 for all the samples separately? If yes, how to do it.

I need them for FRASER (spilicing detction) I would also appreciate if anyone used FRASER before could help me a bit to get started. I am working with plant data for the first time.

Thanks.


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