Hi everyone,
I'm running the TruSight One panel, and the samples look great on Qubit and Bioanalyzer after the 1st PCR. However, at the end of the process I am validating the library and there is nothing there.....well, maybe a TINY little bit, but nothing in terms of the proper amounts.
Any ideas? TechSupport is trying to help, and they say the thermocycler may be the problem. We use the SensoQuest or the Biometra. They are saying the hyb and capture steps are very sensitive, and they have only approved 3 thermocyclers for this use. They say this ramping down step is extremely important, but cannot tell me what specs to test any other PCR machines with. In essence, they are saying it's a guessing game, and to just try multiple machines if we can't buy the recommended cyclers. 1 of the 3 recommended cylcers is now discontinued, 1 of the tetrads is on backorder for 6-8 months, and the last one is extremely expensive.
Does anyone have any experience with non-recommended cyclers?
Thanks,
Jim
I'm running the TruSight One panel, and the samples look great on Qubit and Bioanalyzer after the 1st PCR. However, at the end of the process I am validating the library and there is nothing there.....well, maybe a TINY little bit, but nothing in terms of the proper amounts.
Any ideas? TechSupport is trying to help, and they say the thermocycler may be the problem. We use the SensoQuest or the Biometra. They are saying the hyb and capture steps are very sensitive, and they have only approved 3 thermocyclers for this use. They say this ramping down step is extremely important, but cannot tell me what specs to test any other PCR machines with. In essence, they are saying it's a guessing game, and to just try multiple machines if we can't buy the recommended cyclers. 1 of the 3 recommended cylcers is now discontinued, 1 of the tetrads is on backorder for 6-8 months, and the last one is extremely expensive.
Does anyone have any experience with non-recommended cyclers?
Thanks,
Jim
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