SEQanswers

SEQanswers (http://seqanswers.com/forums/index.php)
-   Literature Watch (http://seqanswers.com/forums/forumdisplay.php?f=10)
-   -   RNA-Seq: The sensitivity of massively parallel sequencing for detecting candidate inf (http://seqanswers.com/forums/showthread.php?t=11575)

Newsbot! 05-24-2011 03:00 AM

RNA-Seq: The sensitivity of massively parallel sequencing for detecting candidate inf
 
Syndicated from PubMed RSS Feeds

The sensitivity of massively parallel sequencing for detecting candidate infectious agents associated with human tissue.

PLoS One. 2011;6(5):e19838

Authors: Moore RA, Warren RL, Freeman JD, Gustavsen JA, Chénard C, Friedman JM, Suttle CA, Zhao Y, Holt RA

Massively parallel sequencing technology now provides the opportunity to sample the transcriptome of a given tissue comprehensively. Transcripts at only a few copies per cell are readily detectable, allowing the discovery of low abundance viral and bacterial transcripts in human tissue samples. Here we describe an approach for mining large sequence data sets for the presence of microbial sequences. Further, we demonstrate the sensitivity of this approach by sequencing human RNA-seq libraries spiked with decreasing amounts of an RNA-virus. At a modest depth of sequencing, viral transcripts can be detected at frequencies less than 1 in 1,000,000. With current sequencing platforms approaching outputs of one billion reads per run, this is a highly sensitive method for detecting putative infectious agents associated with human tissues.

PMID: 21603639 [PubMed - in process]



More...


All times are GMT -8. The time now is 05:43 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2022, vBulletin Solutions, Inc.