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Tsequencing 04-15-2019 04:34 AM

Control-based correction in multispecies RNAseq comparison
Hi all!

I think this issue fits better here than on another forum I posted this question some time ago:

In a multispecies gene expression comparison (per species one control group and 3 treatments) I want to compare the gene expression patterns of specific gene families. For this I want to correct the expression levels of these genes per species using the control group. In this way you get the percentage difference in expression of the genes for each treatment in comparison to the control treatment, and I can compare the gene expression levels of the different species directly instead of only looking at within species patterns and comparing both.

I have not seen a similar approach in other multi-species RNAseq studies. Are there perhaps similar studies or papers dealing with a multispecies comparison of RNAseq data that I have overlooked?

Like to hear your opinion!

Gopo 04-15-2019 05:38 AM

Sounds logical, but not sure. This article might be of interest...


Tsequencing 04-29-2019 08:12 AM

Ah! Thanks for the suggestion, I will give it a try.

INTENSE X 04-29-2019 11:24 AM

thank you will try it

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