Hi,
I have one question regarding microarrays,I was checking Published data, and they published signal intensity values for Affymetrix Probe Ids
I wanted to check the expression of certain genes in that dataset, so I annotated the Affy_Ids to Gene names
Eg:
File Affy_Id Sig.Intensity Gene Species Description
NEB_RA_rep1_annotate.txt: 1428888_at 130.05 tmem33 Mus musculus transmembrane protein 33
NEB_RA_rep1_annotate.txt: 1436028_at 469.84 tmem33 Mus musculus transmembrane protein 33
NEB_RA_rep2_annotate.txt: 1428888_at 104.99 tmem33 Mus musculus transmembrane protein 33
NEB_RA_rep2_annotate.txt: 1436028_at 420.72 tmem33 Mus musculus transmembrane protein 33
NEB_RA_rep3_annotate.txt: 1428888_at 67.26 tmem33 Mus musculus transmembrane protein 33
NEB_RA_rep3_annotate.txt: 1436028_at 290 tmem33 Mus musculus transmembrane protein 33
I see that for each condition, there are two Affy_Ids annotating to the same gene , with different intensity values. Its confusing to judge the intensity value for a gene in particular condition..
Any suggestions??
I have one question regarding microarrays,I was checking Published data, and they published signal intensity values for Affymetrix Probe Ids
I wanted to check the expression of certain genes in that dataset, so I annotated the Affy_Ids to Gene names
Eg:
File Affy_Id Sig.Intensity Gene Species Description
NEB_RA_rep1_annotate.txt: 1428888_at 130.05 tmem33 Mus musculus transmembrane protein 33
NEB_RA_rep1_annotate.txt: 1436028_at 469.84 tmem33 Mus musculus transmembrane protein 33
NEB_RA_rep2_annotate.txt: 1428888_at 104.99 tmem33 Mus musculus transmembrane protein 33
NEB_RA_rep2_annotate.txt: 1436028_at 420.72 tmem33 Mus musculus transmembrane protein 33
NEB_RA_rep3_annotate.txt: 1428888_at 67.26 tmem33 Mus musculus transmembrane protein 33
NEB_RA_rep3_annotate.txt: 1436028_at 290 tmem33 Mus musculus transmembrane protein 33
I see that for each condition, there are two Affy_Ids annotating to the same gene , with different intensity values. Its confusing to judge the intensity value for a gene in particular condition..
Any suggestions??
Comment