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-   -   MiSeq v3 kit for high diversity vs. low diversity libraries (

phyllosphere 12-22-2015 01:56 PM

MiSeq v3 kit for high diversity vs. low diversity libraries
Like many folks working on low diversity libraries, we have been hit hard by the problems associated with the V3 kit - lots of time and effort wasted. Is this problem also common for high diversity libraries? I would appreciate any insights.

microgirl123 12-23-2015 06:01 AM

I've had problems with high-diversity (gDNA) libraries with the 600-cycle V3 kits, even with a cluster density of 1K. Read 2 just tanked after about 50 bp. We are advising people not to run them at all until Illumina sorts out whatever problem this is.

phyllosphere 12-23-2015 06:43 AM

Thank you! Will it be possible to mix our MiSeq libraries with our HiSeq libraries (e.g., RNA-Seq, have not started yet) and running the mix libraries with HiSeq2500 or 3000?

Jessica_L 12-23-2015 08:34 AM

I've run libraries on the MiSeq (usually for troubleshooting purposes) and then loaded them on a HiSeq once I was sure everything was okay with them. The loading concentrations are usually different, in my experience, but that should be the only issue you'll need to address.

phyllosphere 12-23-2015 12:29 PM

Good to know-thanks!

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