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Fonz 10-23-2008 05:10 AM

Barcode of plants
 
Dear All,

part of my work will be a study on the cpDNA for barcoding of plants, I need a variable region within conserved primers. I think I might look for a conserved gene who presents (in the nucleotide sequence) an intron in within the active site of the protein... does anyone know a chloroplast gene who responds to my requests?

cheers

Alfonso

Fonz 10-24-2008 01:41 AM

cpDNA extraction
 
dear all, I have one more question, I do not have in the labs here the instruments and the expertize to carry a cpDNA extraction (it seems that an ultracentrifuge is needes according to the protocol of jansens et al 2005). So I should send my samples to someone who extract cpDNA as a routine work. Could you give me any advice?
Sincerely
Fonz


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