I was wondering if anyone has had any issues sequencing samples that were prepped using a micrococcal nuclease?
I ran a flow cell that contained mostly these types of samples and the T channel was off the charts during the 1st few bases which basically made it look like everything was over clustered; even though I was running these samples at 10pM, which is my norm.
Does anyone have any experience sequencing these types of samples on the Illumina GAIIx?
Thanks!
I ran a flow cell that contained mostly these types of samples and the T channel was off the charts during the 1st few bases which basically made it look like everything was over clustered; even though I was running these samples at 10pM, which is my norm.
Does anyone have any experience sequencing these types of samples on the Illumina GAIIx?
Thanks!