SEQanswers

SEQanswers (http://seqanswers.com/forums/index.php)
-   Illumina/Solexa (http://seqanswers.com/forums/forumdisplay.php?f=6)
-   -   Paired End Protocol for Very Low Abundance Samples (http://seqanswers.com/forums/showthread.php?t=8410)

Daytwa 12-14-2010 05:10 PM

Paired End Protocol for Very Low Abundance Samples
 
All,

This is my first post after sometime lurking. Utilizing what I've seen in the forum I think I have suitably adapted the standard Illumina ChIP paired end protocol to achieve our goal of generating a sequencing library from 0.3 -0.5 ng input. The changes are not surprising (reduced vol. ligation step, diluting adapters, 20 cycles PCR enrichment, etc).

Has anyone come across a reliable protocol or perhaps have any other suggests. Thanks everyone.

saikumarkv 12-15-2010 07:46 AM

I tried to respond directly, but your were not accepting emails. Yes the final conc of PE adapters I used for 1ng was 1.67nM.
Sai

seqgirl123 01-09-2011 01:13 PM

would either of you please share your protocol with me? I have been thinking of ways to make PE libraries from low input DNA (in ng). I will really appreciate it.


All times are GMT -8. The time now is 06:34 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.