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-   -   Demultiplexing MiSeq Runs with Miseq Reporter (http://seqanswers.com/forums/showthread.php?t=49018)

cinimod 12-17-2014 04:37 AM

Demultiplexing MiSeq Runs with Miseq Reporter
 
By default, MiSeq Reporter allows up to 1 mismatch when demultiplexing an indexed MiSeq run. Can I force the software to perform exact matches while demultiplexing the run? Is there an option that can be set in the sample sheet, for example?

GenoMax 12-17-2014 06:24 AM

Looking at the MiSeq reporter manual it does not look like there is a way to do that. Have you tried to contact illumina tech support (this may be a non-documented feature)?

cinimod 12-17-2014 09:58 PM

Thanks for your help, according to the Illumina tech support it is not possible. This is the answer: "MSR demultiplexes with a mismatch of 1. This is not configurable. If you'd like to demultiplex with a different mismatch, I'd advise exploring bcl2fastq1.8.4."


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