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-   -   Randomly vanishing / extremely small G and C Peaks in Sequence (http://seqanswers.com/forums/showthread.php?t=64627)

mikebravo 12-02-2015 12:54 AM

Randomly vanishing / extremely small G and C Peaks in Sequence
 
Hi all,

I want to ask if anyone ever experienced randomly small G and C peaks in their sequence. We are using BDT1.1 on a AB3130 and AB310 sequencer. Since a while we observe every here and than very tiny G and C peaks in an otherwise normal and good sequence. This is reproducible on both sequencers. Sometimes the basecaller even regards it as a gap/deletion.
- Changing the LOT of the BDT1.1 did not help.
- Different PCR-Kits and conditions did not help
- Same observation on two sequencers.
- Changing the sequncing cycle programm did not help.

https://drive.google.com/file/d/0B1U...ew?usp=sharing


I am thankful for any idea.

-mike-


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