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-   -   Viral genome assembly from RNA-seq data (http://seqanswers.com/forums/showthread.php?t=22203)

mcastro 08-02-2012 07:21 AM

Viral genome assembly from RNA-seq data
 
Hi,

I'm performing a RNA-seq analysis from a virus infected plant. After mapping the Illumina Hiseq paired reads against the plant genome, I used the unmapped reads to blast the virus genome available. Once I separated virus reads from other unmapped reads I would like to assembly the virus genome. Which is the best way to do it? I used before velvet and trinity for eukaryote transcriptome assemblies but I'm not sure if I can use the same software or approach for virus.

I would appreciate someone could guide me.

Thanks a lot

dramit1623 11-14-2015 11:29 PM

Dear,

generally i used bowtie first then samtools to generate mapped reads and then use Macvector software to assemble the genome.

thanks.

blancha 11-15-2015 06:21 AM

This is a very naive question, but how can you be sure that the unmapped reads belong to a virus?

Isn't it just as likely that they belong to unmapped regions of the plant transcriptome, sequencing errors, ...?


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