SEQanswers

SEQanswers (http://seqanswers.com/forums/index.php)
-   General (http://seqanswers.com/forums/forumdisplay.php?f=16)
-   -   Proper way to prepare 5M Betaine monohydrate without oxidation? (http://seqanswers.com/forums/showthread.php?t=49099)

KJoseph 12-19-2014 05:11 AM

Proper way to prepare 5M Betaine monohydrate without oxidation?
 
Hello,

One of our buffers uses a stock of 5M betaine in which my lab prepares from dried betaine monohydrate. The buffer contains betaine, 10mm Tris-HCl, Tween 20, and water. We've tried DEPC water and Milli-Q water, but for whatever reason, our oxidation scores keep coming back bad.

When we use pre-made 5M betaine (like from Affymetrix), the oxidation scores look great!

Can anyone provide some suggestions on the proper procedure for preparing non-oxidized 5M betaine?

KJoseph 12-19-2014 05:37 AM

Currently, we put the betaine under heat until it goes into solution with stirring.


All times are GMT -8. The time now is 12:08 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.