Hi There,
I am new to handling genomic DNA so have certain experimental doubts:
1. When we prepare certain dilution of genomic DNA, how do we make sure that genomic DNA solution is homogenously mixed. Is gentle pipetting enough or we should heat the sample at aroung 40-45 C (104-113 F)?
2. What is the procedure of reconsituting powdered form of genomic DNA, can we add buffer and heat it at 65C (149F) to dissolve it properly?
Early response would help me a lot.
Thanks!
I am new to handling genomic DNA so have certain experimental doubts:
1. When we prepare certain dilution of genomic DNA, how do we make sure that genomic DNA solution is homogenously mixed. Is gentle pipetting enough or we should heat the sample at aroung 40-45 C (104-113 F)?
2. What is the procedure of reconsituting powdered form of genomic DNA, can we add buffer and heat it at 65C (149F) to dissolve it properly?
Early response would help me a lot.
Thanks!
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