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wei210 10-08-2019 09:26 PM

Kapa lib quant question
 
Hello. I am new here. I have a naive question. When we make series dilution for kapa lib quant.
1) Will it make big difference if dilute the lib using H2O vs 10mM tris PH8.0 w 0.05% tween-20 ?
2) will kapa conc typically higher than the Qubit conc (lib w Illumina p5/p7 sequence primer) ?
3) vetex to mix should be better than Pipet up/down. In the case using Pipet up/down. How many times up/down should be sufficient?
Thank you all so much

seq198 10-10-2019 02:11 AM

For PCR-free libs kapa quant kit makes sense, as qubit will not deliver accurate results. he different buffers for diluting should not have a big effect for what I can say. 5 times pipetting up and down should be sufficient.

wei210 10-10-2019 06:18 AM

Thank you so much, Seq198


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