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-   -   ampure beads (http://seqanswers.com/forums/showthread.php?t=28236)

sc10021 03-08-2013 10:50 AM

ampure beads
 
Left my beads on bench overnight. Is it still OK to use?

kcchan 03-10-2013 09:09 PM

The performance shouldn't be affected from sitting at room temperature overnight. The beads are just magnets in polyethylene glycol; there's nothing in terms of enzymes or anything that would degrade. As long as you don't boil them or freeze them they'll last a while.

eab 03-12-2013 07:59 PM

only potential problem is stuff growing in them and that stuff's DNA getting into whatever library you were making. not a problem for all library prep protocols.

sc10021 03-12-2013 10:08 PM

Thank you.

elhb 08-11-2014 07:41 AM

Hi, what do you think might happen if you by chance happen to freeze them??

eab 08-11-2014 07:43 AM

i frequently freeze beads under water sup during elution and then thaw, incubate, and remove beads later

if you freeze the raw ampure solution i'm not sure what happens, it's pretty concentrated stuff so there might be some precipitation. or maybe not. if not i bet they're fine.

kerplunk412 10-23-2014 03:13 PM

Quote:

Originally Posted by elhb (Post 147264)
Hi, what do you think might happen if you by chance happen to freeze them??

I have always heard that freezing magnetic beads in general makes them behave "weird," but I don't recall any actual details or evidence.

Leaving AMPure XP beads on your bench overnight will not affect the performance at all. I have known people who stored beads at room temp for weeks and used them regularly with no problems, although I wouldn't recommend that.

IdahoRAD 10-30-2014 03:47 PM

I have personally left Ampure beads out, ON, on accident. Paranoid, I thought I couldn't use them, but I did, and everything was just fine!

hssalgh2 06-28-2017 10:27 AM

hello
i was wondering about left Ampure by chance the beads at Room temperature for two weeks and i don't know if it still works or not and it costs me around 700 pounds?
please help me or any suggestions that would be appreciated.

thank you very much

kerplunk412 06-28-2017 10:37 AM

Quote:

Originally Posted by hssalgh2 (Post 208755)
hello
i was wondering about left Ampure by chance the beads at Room temperature for two weeks and i don't know if it still works or not and it costs me around 700 pounds?
please help me or any suggestions that would be appreciated.

thank you very much

The beads should be fine.

Simone78 07-01-2017 05:52 AM

Quote:

Originally Posted by hssalgh2 (Post 208755)
hello
i was wondering about left Ampure by chance the beads at Room temperature for two weeks and i don't know if it still works or not and it costs me around 700 pounds?
please help me or any suggestions that would be appreciated.

thank you very much

There are many protocols for producing "home-made" Ampure beads . The one I follow (Rohland & Reich, Genome Res 2012) uses NaN3 (sodium azide) as preservative at a final conc of 0.05%. Ampure beads are SO expensive, I do hope that Beckman is also using NaN3 to increase their shelf life! They simply must do it :)

hssalgh2 07-01-2017 06:21 AM

Quote:

Originally Posted by Simone78 (Post 208855)
There are many protocols for producing "home-made" Ampure beads . The one I follow (Rohland & Reich, Genome Res 2012) uses NaN3 (sodium azide) as preservative at a final conc of 0.05%. Ampure beads are SO expensive, I do hope that Beckman is also using NaN3 to increase their shelf life! They simply must do it :)

Thank you so much but is it ok when I left beads on the shelf for two weeks not in the friadg that happen by chance!

Regards

torben 07-03-2017 01:26 AM

Quote:

Originally Posted by Simone78 (Post 208855)
There are many protocols for producing "home-made" Ampure beads . The one I follow (Rohland & Reich, Genome Res 2012) uses NaN3 (sodium azide) as preservative at a final conc of 0.05%. Ampure beads are SO expensive, I do hope that Beckman is also using NaN3 to increase their shelf life! They simply must do it :)

A qucik look at the SDS (https://www.beckmancoulter.com/wsrpo...entId=45481385 ) confirms that they do.


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