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-   -   Oligos on the flowcell -- how'd they get there? (http://seqanswers.com/forums/showthread.php?t=14959)

MrGuy 10-22-2011 02:36 AM

Oligos on the flowcell -- how'd they get there?
 
Hi Guys, dumb question, maybe it's already been answered and I can't really find any relevant information via google:

How does Illumina attach the oligos to the surface of the flowcell? Or, generally, how does one attach oligos to the surface of a flowcell?

Thanks in advance...

csquared 10-31-2011 11:57 AM

Its all detailed in the Bentley et al Nature paper from a couple years ago. In the Supplemental material. Don't know if the same procedure is used today, but it does describe the creation of the flow cell surfaces.


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