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arunkh 02-19-2013 09:01 PM

Problems in Truseq library prep

We were preparing few truseq libraries for exome capture but after PCR enrichment found out that the yield obtained is very low and cannot proceed with exome capture. Strange part is for some of the samples in this batch exome capture was done successfully earlier and now it doesn't seem to work. Cross checked and went through the protocols, checked all reagent expiry dates still couldn't figure out what could have gone wrong this time. No changes in the protocol whatsoever was done. Can someone help us in figuring out this issue?

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