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-   -   Optimized library prep solution for ctDNA and cfDNA (http://seqanswers.com/forums/showthread.php?t=44378)

Bioo Scientific 06-20-2014 11:38 AM

Optimized library prep solution for ctDNA and cfDNA
 
The new NEXTflex Cell Free DNA-Seq Kit is optimized to produce libraries from 1 ng of circulating tumor DNA (ctDNA) or cell free fetal DNA (cffDNA), in two hours or less. This low-input library preparation kit delivers high coverage quality and reduced bias for Illumina sequencing applications. The NEXTflex Cell Free DNA-Seq Kit contains a high fidelity enzyme which exhibits minimal GC bias and produces uniform coverage of difficult to sequence DNA. The patent pending Enhanced Adapter Ligation Technology incorporated into the ligation step offers the highest ligation efficiency available, resulting in greater library diversity and a larger number of unique sequencing reads. Up to 192 unique adapter barcodes are available, facilitating high-throughput multiplexing. Protocols for automating library prep using the NEXTflex Cell Free DNA-Seq Kit are also available, for Bioo Scientific's SEQBOT™ NGS Library Prep Automation Platform, the Beckman BiomekŪ FX and BiomekŪ FXP Laboratory Automation Workstations, and the Sciclone NGS, Sciclone NGSx and Sciclone G3 platforms.

Bioo Scientific 10-15-2014 11:25 AM

The NEXTflex Cell Free DNA-Seq Kit is designed for making NGS libraries from low-input cfDNA or ctDNA samples. Visit our website for data demonstrating the use of this kit to produce high-quality informative libraries from cell-free DNA extracted from plasma.

nucacidhunter 10-15-2014 12:52 PM

Do you have any data that demonstrates superiority of your ligation technology in comparison to other similar products? And can you explain to your potential customers why your library protocols are not freely avaible online like other vendors?

sequencingfan 11-05-2014 12:32 AM

I have question. Is nextflex compatible with Ion proton sequencer?Do You provide adapters and other components separetly?


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