I am new to NGS, I have just received sequenced raw files of non-model plant from Hiseq. I would like to know sequence quality check for quality filtering. I also like to do K-mer analysis and heterozygosity rate analysis. Please let me know the best tools to do this.
I have selected these workflow/tools, better workflow/tool recommendation are welcome.
raw reads->Sequence quality check - FastQC->K-mer analysis- Jellyfish ->to estimate genome size & Heterozygosity rate analysis- GCE (genomic charactor estimator)
I have selected these workflow/tools, better workflow/tool recommendation are welcome.
raw reads->Sequence quality check - FastQC->K-mer analysis- Jellyfish ->to estimate genome size & Heterozygosity rate analysis- GCE (genomic charactor estimator)
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