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-   -   Fast flow cells and Allpaths (http://seqanswers.com/forums/showthread.php?t=34985)

troendle 10-31-2013 09:29 AM

Fast flow cells and Allpaths
 
Hi everyone. I'm working on a small fish genome and want to know if anyone is having any success using the new fast flow cells for HiSeq that give >100bp reads as input along with a 3000bp mate pair read as the input for allpaths rather than making the 180bp library. The fast flow cells generate single reads rather than paired reads which is why I'm not sure if this will work. Any input is appreciated especially if you have tried this.

GenoMax 10-31-2013 10:16 AM

Quote:

Originally Posted by troendle (Post 120488)
The fast flow cells generate single reads rather than paired reads which is why I'm not sure if this will work.

That is not correct. Rapid mode flowcells can generated paired-end reads.

troendle 10-31-2013 11:00 AM

Yes they can produce paired end reads but the library that we are attempting to work from is a 450bp library so they would not be overlapping. What we want to know is if allpaths can handle non-paired reads, or if we need to go ahead and make the 180bp library.

GenoMax 10-31-2013 12:16 PM

Ok. Your original post was not clear.

You may want to consider running on MiSeq with the new v.3 kits. That can give you 2 x 300 bp reads.


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