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-   -   RQ1 DNase I digestion of Total RNA (http://seqanswers.com/forums/showthread.php?t=64874)

vaslanzadeh 12-14-2015 12:55 PM

RQ1 DNase I digestion of Total RNA
 
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I am having problems with the quality of RNA after RQ1 DNase I treatment. After DNase treatment, I use phen/Chlor for RNA purification. I use ethanol for final precipitation. If you look at my bioanlyser trace before and after DNase digesition, there is a large pick after ribosomal RNA. I do not know what this is. If I heat the RNA 6 minutes at 65, I do not see this extra pick but the quality of the RNA drops a lot. I am worried that this pick can affect the Ribo-Zero step and library Prep.

Does anybody had this problem?

Thanks


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