Recently I notice that the new version of Tophat does not output the read pairs which map to two different chromosomes. However, these alignments are outputted by old version. Does anyone know an option to get them out from the new Tophat?
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That is a bit scary. I wonder if this is a design shift with the introduction of tophat-fusion which is design for this issue. More I'm worried that a wierd cufflinks abundance issue with more "FAIL" genes might be related to a tophat error not cufflinks. Time for a matrix comparison of previous and current tophat version with previous and current cufflinks versions.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...-
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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