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anyone1985 09-06-2009 01:20 AM

blast puzzle
When I ran the blast with the parameter of "-b 1 -v 1", the result still had several subjects. This puzzle has puzzled me long time.

rglover 09-06-2009 02:45 AM

It could be that you're getting multiple hsp's for the same hit. You're restricting it to 1 hit and 1 alignment, but each hit can have multiple hsps, although I'm not sure how to restrict that, if it's even possible at all (I'm sure someone more knowledgeable than me will have the answer to that!).

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