Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Custom Yeast Genomic 70-mer Oligonucleotide Microarray chip NCBI GEO dataset?

    How to extract the numerical values for plotting time series curves for this Custom Yeast Genomic 70-mer Oligonucleotide Microarray chip NCBI GEO dataset?
    How to extract the numerical expression values for plotting time series curves for this Custom Yeast Genomic 70-mer Oligonucleotide Microarray NCBI GEO dataset?
    I would like to plot the mRNA expression time series curves based on the data of the following NcBI GEO dataset.
    Series GSE3705
    The title of this dataset is:
    JM43 cells grown aerobically in galactose and treated with Antimycin A
    The URL of this dataset is:
    NCBI's Gene Expression Omnibus (GEO) is a public archive and resource for gene expression data.

    The citation of the publication, which goes along with this dataset, is:
    Lai LC, Kissinger MT, Burke PV, Kwast KE. Comparison of the transcriptomic "stress response" evoked by antimycin A and oxygen deprivation in Saccharomyces cerevisiae. BMC Genomics 2008 Dec 23;9:627. PMID: 19105839.
    This publication is free available at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2637875/
    My problem is that the platform is

    GPL1535 Custom Yeast Genomic 70-mer Oligonucleotide Microarray
    What do I need to do to extract the numerical gene expression values from this dataset, which are most meaningful for plotting time series curves?
    Thanks a lot
    Thomas

Latest Articles

Collapse

  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM
  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
25 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
27 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 09:21 AM
0 responses
24 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-04-2024, 09:00 AM
0 responses
52 views
0 likes
Last Post seqadmin  
Working...
X