Seeing a read like this from 454 Titanium shotgun experiment using DNA from a capture array.
tcagCTCGAGATTCTGGATCCTCACGTAATTCATCCTACATTACCTAGTAATTggtgaccatctgcattagctaattagcttatagaagaagacaacttctcatggtttatgacagaatata
gtctgcaacttggagcaaggcacacaggggattaggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn
first tcag are the key sequence. Why is the rest of the sequence in upper and lower case? Thought the upper case meant good quality bases, but looking at the .fna files this does not seem to be the case.
Any help to understand this is appreciated
Cheers
L
tcagCTCGAGATTCTGGATCCTCACGTAATTCATCCTACATTACCTAGTAATTggtgaccatctgcattagctaattagcttatagaagaagacaacttctcatggtttatgacagaatata
gtctgcaacttggagcaaggcacacaggggattaggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn
first tcag are the key sequence. Why is the rest of the sequence in upper and lower case? Thought the upper case meant good quality bases, but looking at the .fna files this does not seem to be the case.
Any help to understand this is appreciated
Cheers
L
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