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Hello,
I have 27 samples of total RNA isolated from E. coli which (having passed all initial requirements) have failed QC prior to sequencing. We are using a HiSeq 2 x 150 bp reads. The RINs are fine but the DV200 is low- the company have said this indicates degradation but looking at the tapestation trace it doesnt seem to be degraded. The large band of what I am presuming are tRNA is skewing the DV200. My main concern is considering we are mainly interested in the mRNA should we proceed with sequencing considering the large abundance of tRNA?
I have 27 samples of total RNA isolated from E. coli which (having passed all initial requirements) have failed QC prior to sequencing. We are using a HiSeq 2 x 150 bp reads. The RINs are fine but the DV200 is low- the company have said this indicates degradation but looking at the tapestation trace it doesnt seem to be degraded. The large band of what I am presuming are tRNA is skewing the DV200. My main concern is considering we are mainly interested in the mRNA should we proceed with sequencing considering the large abundance of tRNA?