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-   -   genome assembly polisher that can use Sanger reads? (http://seqanswers.com/forums/showthread.php?t=88270)

ssully 03-20-2019 11:25 AM

genome assembly polisher that can use Sanger reads?
 
We have a PacBio final assembly that requires some polishing to fix e.g. erroneous stop codons. No Illumina reads at hand but we do have a set of high quality Sanger reads from an older version of the genome. Is there a tool like Racon but can use Sanger reads to correct small errors in an assembly?

JTH-Genome 06-27-2019 10:55 AM

You could try to modify one of these error correction algorithms: http://wgs-assembler.sourceforge.net...index.php/PBcR


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