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-   -   Comparison of NGS analyser tech info (http://seqanswers.com/forums/showthread.php?t=3576)

gavin.oliver 01-11-2010 12:01 AM

Comparison of NGS analyser tech info
 
Hi all,

I'm sure this question has been asked before so maybe I'll just be pointed to an old thread, but:

Basically I'm looking for an up-to-date comparison of the major NGS analysers' main technical info - perhaps in table format?

Thanks in advance,

Gavin

gavin.oliver 01-12-2010 12:31 AM

Does noone have any ideas on this? :confused:

It would be aprticularly useful to get an idea of the cost per run of the major contenders (454, Solid, Solexa).

Thanks in advance :)

gavin.oliver 01-12-2010 05:01 AM

I found this comprehensive comparison:

http://genomics.ucr.edu/about/report...1207_Table.pdf

Can someone advise whether any of the info is very outdated? I think the document is from March 2008.

Thanks :D

ffinkernagel 01-12-2010 05:36 AM

there's http://www.clcngs.com/2008/12/ngs-platforms-overview/ which has some about everything but the price per base.

gavin.oliver 01-12-2010 05:46 AM

Really useful, thanks :)

I'm a newbie so forgive me for asking - Do the Solid 1 and 2 and Solexa GA and GA II represent old (outdated) models?

ffinkernagel 01-12-2010 05:57 AM

Not being immensely familiar with them myself, I know the GAIIx is an upgrade to the GAII machine, and I presume the GA is an older machine.

Please note that the list always contains the 'largest run' - ie. you can run a GAIIx with only 36 bp which makes a single run considerably faster (but in the end I guess is about the same/a little less throughput, but lower latency)

VanessaS 01-28-2010 06:04 PM

Quote:

Originally Posted by gavin.oliver (Post 12281)
Really useful, thanks :)

I'm a newbie so forgive me for asking - Do the Solid 1 and 2 and Solexa GA and GA II represent old (outdated) models?


I can only speak about the SOLiD, 1 and 2 are old versions. They have come out with the 3, the 3+, and in a month or two will bring out 4. By the end of the year (we shall see) 4hq. Times are a changin'!

gavin.oliver 01-29-2010 02:14 AM

I'm a bit confused about the accuracies. Some sources suggest Illumina is more accurate than ABI and some suggest the opposite. The specs on their respective websites would seem to suggest that the SOLiD is currently the most accurate when running at full capacity.

avilella 01-29-2010 11:26 PM

Next Generation Sequencing spreadsheet
 
Hi all,

I've generated a spreadsheet with the numbers I've compiled from different blogs and news announcements.

http://spreadsheets.google.com/ccc?k...hQTHc&hl=en_GB

If anyone has any corrections or knows any other systems or data points, I've made editing public.

If you want to embed it to a webpage, you can copy+paste the following link:

<iframe width='500' height='500' frameborder='0' src='http://spreadsheets.google.com/pub?key=twmPdmhilecE5bex4WiHPLw&single=true&gid=0&range=A2%3AR25&output=html&widget=true'></iframe>

Xi Wang 01-30-2010 02:36 AM

Quote:

Originally Posted by avilella (Post 13245)
Hi all,

I've generated a spreadsheet with the numbers I've compiled from different blogs and news announcements.

http://spreadsheets.google.com/ccc?k...hQTHc&hl=en_GB

If anyone has any corrections or knows any other systems or data points, I've made editing public.

Oh my god, I can't access google spreadsheet from China..

KevinLam 03-19-2010 02:41 AM

Quote:

Originally Posted by avilella (Post 13245)
Hi all,

I've generated a spreadsheet with the numbers I've compiled from different blogs and news announcements.

http://spreadsheets.google.com/ccc?k...hQTHc&hl=en_GB

If anyone has any corrections or knows any other systems or data points, I've made editing public.

If you want to embed it to a webpage, you can copy+paste the following link:

<iframe width='500' height='500' frameborder='0' src='http://spreadsheets.google.com/pub?key=twmPdmhilecE5bex4WiHPLw&single=true&gid=0&range=A2%3AR25&output=html&widget=true'></iframe>

Nice work!

RockChalkJayhawk 03-19-2010 07:04 AM

Cost per run of different platforms
 
I'm trying to get a rough idea of how much it costs every time you turn on one of these instruments. Can you all help approximate? I know it vaires on application, so a range is acceptable.

I know the HiSeq is ~$20K (10K if just one flow cell), but what about
GAIIe:
SOLiD4:
SOLiD PI:

?

ymc 03-22-2010 06:36 PM

Quote:

Originally Posted by Xi Wang (Post 13247)
Oh my god, I can't access google spreadsheet from China..

That's why BGI set up shop in Hong Kong. ;)

ymc 03-22-2010 06:39 PM

Quote:

Originally Posted by avilella (Post 13245)
Hi all,

I've generated a spreadsheet with the numbers I've compiled from different blogs and news announcements.

http://spreadsheets.google.com/ccc?k...hQTHc&hl=en_GB

If anyone has any corrections or knows any other systems or data points, I've made editing public.

If you want to embed it to a webpage, you can copy+paste the following link:

<iframe width='500' height='500' frameborder='0' src='http://spreadsheets.google.com/pub?key=twmPdmhilecE5bex4WiHPLw&single=true&gid=0&range=A2%3AR25&output=html&widget=true'></iframe>

Is this really correct? I think on Illumina's official page, HiSeq 2000 is max 200Gb/run and GAIIx is max 50Gb/run.

Xi Wang 03-22-2010 06:40 PM

Quote:

Originally Posted by ymc (Post 15833)
That's why BGI set up shop in Hong Kong. ;)

So does google...

ymc 03-22-2010 07:16 PM

Quote:

Originally Posted by Xi Wang (Post 15836)
So does google...

Yeah, that too. But to stay on topic, 100 HiSeq 2000s will be in HK. This will make HK the sequencing capital of the world! :cool:

Chipper 03-23-2010 02:29 AM

Quote:

Originally Posted by ymc (Post 15834)
Is this really correct? I think on Illumina's official page, HiSeq 2000 is max 200Gb/run and GAIIx is max 50Gb/run.

http://www.genomeweb.com/sequencing/...new-instrument

ymc 03-23-2010 05:36 AM

Quote:

Originally Posted by Chipper (Post 15855)

Do we really need 60x coverage for genome sequencing???:confused:

nickloman 03-23-2010 08:54 AM

Quote:

Originally Posted by ymc (Post 15840)
Yeah, that too. But to stay on topic, 100 HiSeq 2000s will be in HK. This will make HK the sequencing capital of the world! :cool:

Who in HK is buying 100 HiSeq instruments? I did read the press release that BGI are buying 128 HiSeqs but they are based in Shenzhen, which is in mainland China?

nickloman 03-23-2010 08:57 AM

Oh, I've re-read the press release and I see that they are installing the machines in Hong Kong Science Park:
http://www.genomics.cn/en/news_show....pe=show&id=500


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