SEQanswers

SEQanswers (http://seqanswers.com/forums/index.php)
-   454 Pyrosequencing (http://seqanswers.com/forums/forumdisplay.php?f=5)
-   -   Paper: Fully automated process for barcoded libraries for 454 (http://seqanswers.com/forums/showthread.php?t=4072)

RCJK 02-16-2010 08:39 PM

Paper: Fully automated process for barcoded libraries for 454
 
Check this out for automated 454 general library prep:

http://genomebiology.com/2010/11/2/R15

"A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454"

nickloman 02-17-2010 07:41 AM

Very cool. I wish we could just buy it off the shelf and have it working tomorrow!

dottomarco 02-19-2010 09:14 AM

This is based on the old library protocol right? Now the new rapid library protocol solved a lot of issues.. but it wold be interesting to apply the 96 parallelization idea of the paper to the new rapid protocol

james hadfield 06-23-2010 05:46 AM

Fluidigm have a 454 amplicon prep method that allows you to make 48 libraries of up to 480 amplicons, barcoded and ready to sequence. All from one PCR.

nickloman 06-23-2010 08:17 AM

Oh yes, this thing? http://www.fluidigm.com/pr/2010/2010...essRelease.htm

Looks really handy for some of our apps.


All times are GMT -8. The time now is 04:02 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.