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buthercup_ch 08-01-2016 12:38 AM

RiboZero kits
 
Hello,

After looking on the web, I haven't been really successful in finding information about the difference among the several rRNA removal kits from Epicentre (I think it was recently absorbed by Illumina).

Mainly interested in what are the specifications and differences among the following kits:
- Ribo-Zero™ Magnetic Gold Kit (Epidemiology)
- Ribo-Zero™ rRNA Removal Kits (Bacteria)
- Ribo-Zero™ rRNA Removal Kits (Gram-Negative Bacteria)
- Ribo-Zero™ rRNA Removal Kits (Gram-Positive Bacteria)

Thanks in advance

Cristina

Olaf Blue 08-01-2016 05:40 AM

The main difference is the specificity of the rRNA removal solutions in the kits.

Epidemiology = Human/Mouse/Rat plus Gram negative and Gram positive rRNA capture probes
Bacteria = Gram Negative and Gram Positive rRNA capture probes
Gram Negative = only Gram negative bacteria rRNA capture probes
Gram Positive = only Gram Positive bacteria rRNA capture probes

Otherwise the remaining reagents (the magnetic bead kits and the other reagents in the Capture probe modules) are the same.

Olaf


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