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-   -   custom indexed primers for small RNA library (http://seqanswers.com/forums/showthread.php?t=70931)

sunitha 08-18-2016 01:54 PM

custom indexed primers for small RNA library
 
Hi all

I am currently using Illumina kit A and kit B for making small RNA libraries. We need two more indexed primers from kit C to complete our sample set. Could anyone let me know the concentration of the indexed primers Illumina supply so I can custom synthesize it through other vendors. I do not foresee an usage for the entire kit C. So it is a huge investment to buy the whole kit for just two indexed primers.

kerplunk412 08-19-2016 12:02 PM

Quote:

Originally Posted by sunitha (Post 197927)
Hi all

I am currently using Illumina kit A and kit B for making small RNA libraries. We need two more indexed primers from kit C to complete our sample set. Could anyone let me know the concentration of the indexed primers Illumina supply so I can custom synthesize it through other vendors. I do not foresee an usage for the entire kit C. So it is a huge investment to buy the whole kit for just two indexed primers.

Hi Sunitha,
I do not know the concentration of the primers in the Illumina kit, but you can probably estimate it fairly accurately using a Nanodrop or Qubit ssDNA assay.
FYI, you can get indexed primers from Bioo Scientific that are compatible with the TruSeq small RNA library prep kit. To find out the concentration just send an email to BiooNGS@biooscientific.com.

As a disclaimer, I work for Bioo Scientific.

sunitha 08-19-2016 02:13 PM

Thank you very much.


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