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-   -   miRNA's expression normalization (http://seqanswers.com/forums/showthread.php?t=16054)

Giorgio C 12-06-2011 10:54 AM

miRNA's expression normalization
 
Hi everybody,

i have two set of miRNAs: A|B : The first has obtained 1/3 of the total sequences of the second. How do i need to consider the level expression ?

For example if i have 2000 reads for a particular miRNA X in the set A and 1000 in the B it means that miRNA X is about 2x expressed in A respect to B?

If is it like i think i should have a normalization....

What you suggest ?? Is there any way, script, tools that may help ???

Thank you very much...


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