Dear all
I am analyzing gut samples from fish using illumina's 16S protocol, amplifying the v4 hypervariable region. I am getting ok bands after the first PCR step, and a nice peak on the agilent bioanalyzer (approx. 350bp). I am wondering however, after purification of products, what is an acceptable concentration for each sample? I am getting concentrations of individual products of between 0.5 and 9ng/ul, with the average being around 2-4ng/ul per sample. Is this an acceptable concentration to proceed with?
Thank you very much in advance.
Kind regards
Egansbay
I am analyzing gut samples from fish using illumina's 16S protocol, amplifying the v4 hypervariable region. I am getting ok bands after the first PCR step, and a nice peak on the agilent bioanalyzer (approx. 350bp). I am wondering however, after purification of products, what is an acceptable concentration for each sample? I am getting concentrations of individual products of between 0.5 and 9ng/ul, with the average being around 2-4ng/ul per sample. Is this an acceptable concentration to proceed with?
Thank you very much in advance.
Kind regards
Egansbay
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