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-   -   Lotsa new toys from Illumina: HiSeq X Five, 3000, 4000, NextSeq 550 (http://seqanswers.com/forums/showthread.php?t=49486)

GW_OK 01-12-2015 10:36 AM

Lotsa new toys from Illumina: HiSeq X Five, 3000, 4000, NextSeq 550
 
Hiseq 4000
Hiseq 3000
Nextseq 550
HiseqX 5

http://www.illumina.com/company/news...newsid=2006979

Brian Bushnell 01-12-2015 11:02 AM

Any idea if the HS4000/5000 are using 4-color or 2-color chemistry?

GW_OK 01-12-2015 11:09 AM

I think that's Nextseq only?
Looks to me like they've just stuck HiseqX-style flowcells in the 2500 system.

Brian Bushnell 01-12-2015 11:21 AM

Except that I'm pretty sure the X-series do use 2-dye chemistry, the same as NextSeq...

GenoMax 01-12-2015 11:28 AM

Quote:

Originally Posted by GW_OK (Post 157860)
I think that's Nextseq only?
Looks to me like they've just stuck HiseqX-style flowcells in the 2500 system.

Possible upgrade path for current 2500 owners?

I am not going to hold my breath though.

GW_OK 01-12-2015 11:53 AM

Quote:

Originally Posted by Brian Bushnell (Post 157863)
Except that I'm pretty sure the X-series do use 2-dye chemistry, the same as NextSeq...

Can't find any literature that says anything other than the Nextseq uses 2 colors. In fact, isn't this why the Nextseq currently has GC problems? Haven't heard anything about that with the HiseqX...

GW_OK 01-12-2015 11:54 AM

Quote:

Originally Posted by GenoMax (Post 157864)
Possible upgrade path for current 2500 owners?

I am not going to hold my breath though.


LOL, just like I was able to upgrade my v2-camera 2500's for the v4 chemistry....

westerman 01-12-2015 12:00 PM

New toys for one person is a funding/migration headache for another. Especially if there is no upgrade path. :-(

ECO 01-12-2015 01:29 PM

Made the title more explicit and promoted to the front page...

SNPsaurus 01-12-2015 01:52 PM

This http://blog.illumina.com/blog/illumi...eq-4000-system says the 4000 uses 4-color.

This http://www.illumina.com/content/dam/...0-2014-057.pdf says that 1 flowcell does 50 transcriptomes per run at 50M reads each, so that is >300M reads per lane. Looks faster as well, if the longest run time is 3.5 days (not sure if that would be for 1x150bp or 2x150bp).

nucacidhunter 01-12-2015 02:06 PM

There are data from PCR-free, RNA-Seq and NRC libraries sequenced on HiSeq 4000 in BaseSpace.

SNPsaurus 01-12-2015 02:35 PM

Heard from someone that the cluster and reagent kits are similar in price to current Hiseq 2000 reagents, which would mean the price per read is quite a bit lower.

HeinKey 01-13-2015 04:56 AM

#SNPsaurus:
I assume it will be 2x 150 bp in 3.5 days.
2.1 to 2.5 billion clusters per flow cell, in a 2x150 bp run is 630 - 750 Gb.
With a speed >200Gb per day a 2x 150 bp run will take 3.5 days.

I wonder if we can upgrade the 2500 V4 machine to a 4000 version...

kmcarr 01-13-2015 05:23 AM

It looks to me that the 3000/4000 only accept 8 lane flow cells (high-output). If true then the trade off for throughput is flexibility.

austinso 01-13-2015 07:01 AM

Quote:

Originally Posted by GenoMax (Post 157864)
Possible upgrade path for current 2500 owners?

I am not going to hold my breath though.

Ditto. I think supporting the patterned flow-cells would require too much of a reworking of the guts...

Seems like they are following the Apple product release model...

AllSeq 01-13-2015 08:43 AM

Quote:

Originally Posted by Brian Bushnell (Post 157863)
Except that I'm pretty sure the X-series do use 2-dye chemistry, the same as NextSeq...

The X series uses the standard 4-dye chemistry. NextSeq is the only one to use 2-dye. (And the one that people seem to be having the most problems with in terms of quality...)

GenoMax 01-13-2015 08:54 AM

Quote:

Originally Posted by austinso (Post 157945)
Seems like they are following the Apple product release model...

More like Intel model. e.g. socket LGA2011 --> LGA2011-v3 (not backwards compatible).

FWOS 01-13-2015 08:57 AM

Pretty sure they will initially only support 2x125 in 3.5 days

Genohub 01-13-2015 07:35 PM

"The 3000 is to the 4000 as the 1000 was to the 2000 and the 1500 to the 2500" Nice summary here: https://biomickwatson.wordpress.com/...00-in-context/

- Genohub

SNPsaurus 01-13-2015 09:18 PM

The one difference with these machines is the throughput. A university facility needed 2 flow cells that could be run independently to help manage a queue, since a paired end run would be almost two weeks (less now). But if most runs are 1-3 days, then that is less of a problem. I suspect the 3000 won't be substantially cheaper so it won't be appealing since you might as well get twice the capacity for a little extra, but some of the other reasons are less important for these machines.


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