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  • Mixture of antibody - ChIP-seq

    Hi,

    If we want to study interaction of two proteins in transciption, can we use mixture of antibody in ChIP-seq experiment? Is there any references that I can read?

    Thanks,

  • #2
    Are you thinking of something similar to a re-ChIP, where you find what DNA is bound to a complex of both proteins? You can do a normal ChIP with one antibody, then after eluting just ChIP that with the 2nd antibody.


    It probably wouldn't make sense to put antibodies for multiple proteins in a single IP reaction; that would not distinguish between DNA bound by one protein, or both.

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