Hi all
We have to design an experiment to sequence the transcriptomes of 2 different cell types to know what genes are differentially expressed.
ABI recommends different Tag numbers (mappable reads) for different applications but they don't specify the exact (atleast approximate) mappable reads required to survey for different applications.
So to study the differentially expressed genes in different cell types what is the recommended mappable reads or tags we need to get a good coverage.
Thanks for the suggestions
We have to design an experiment to sequence the transcriptomes of 2 different cell types to know what genes are differentially expressed.
ABI recommends different Tag numbers (mappable reads) for different applications but they don't specify the exact (atleast approximate) mappable reads required to survey for different applications.
So to study the differentially expressed genes in different cell types what is the recommended mappable reads or tags we need to get a good coverage.
Thanks for the suggestions
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