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-   -   bacterial RNAseq using TruSeq Lib prep (http://seqanswers.com/forums/showthread.php?t=81135)

thiNGS 03-09-2018 05:08 AM

bacterial RNAseq using TruSeq Lib prep
 
Hi all. I am in the process of starting RNAseq from bacterial samples and I am trying to identify the best strategy to do it. Given that bacterial RNA is not poly-adenylated, a random hexamer-based system is essential. Has anyone used the TruSeq stranded library prep kit for bacterial RNA? Or do you have a good kit to recommend? The samples will be run on the MiSeq or NextSeq systems. Any advice to guide me through the magical world of RNAseq will be much appreciated :D

huguesparri 03-13-2018 02:15 AM

We used the Truseq kit succesfully on Bacteria. 2 things are essential:
- DNAse treatment of your RNA.
- Good depletion efficiency for the ribosomal RNA. Ribozero kit are usually doing well.


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