SEQanswers (
-   Illumina/Solexa (
-   -   ATAC-seq library sequencing (

OlgaMikh 03-12-2019 06:56 AM

ATAC-seq library sequencing

I am about to submit my first mouse ATAC-seq libraries for sequencing, but I have doubts regarding the number of libraries to pool in one 100bp paired-end HiSeq2500 Rapid Run.
The Buenostro paper is suggesting >50M mapped reads per sample (in case of human genome), and the Rapid Run, according to Illumina's specifications, should yield "600 million paired end reads":

My question is: does it mean I can pool 12 samples? Or did Buenostro mean 50 million pairs, which means 100 million reads per sample -- meaning I can only pool 6 samples in one run?

Many thanks for helping to resolve this confusion.

luc 03-12-2019 01:19 PM

Hi Olga,

your math seems correct. Six samples to sequence about 50 million library molecules each. Buenrostro et al. generally refer to paired-reads in their papers.
But you can certainly pool more libraries, analyze the data of the first lane, and then sequence additional lanes if needed.

All times are GMT -8. The time now is 07:09 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.