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bsp022 05-15-2013 03:56 AM

Denoising Illumina reads
 
Does anyone have any advice on denoising Illumina reads (specifically 16s reads from a HiSeq)? I've had a look in the literature and most of the denoising algorithms available seem to be aimed at 454 sequencing (and not really applicable to Illumina since they work on flowgram files). The only real possibility seems to be Rosen et al (2012). I would be very interested to hear about anyone's experience using this or any other approach for denoising Illumina data.


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