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-   -   An ide for RNAseq validation with qPCR (http://seqanswers.com/forums/showthread.php?t=34979)

sindrle 10-31-2013 04:14 AM

An ide for RNAseq validation with qPCR
 
Hi!
Just a thought:

1. Make list of all sign. changed genes called from Cuffdiff, edgeR and DESeq. This will be high sensitive I guess, but low specificity.

2. Correlate against clinical characteristica.

3. Validate all genes which sign. correlate with clin. char. with qPCR

Is this totally of?


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