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-   -   Differences in Consensus Sequences (http://seqanswers.com/forums/showthread.php?t=13107)

bhan 08-01-2011 08:36 AM

Differences in Consensus Sequences
 
Hello everyone,

I'm trying to automate some data collection, and I need a consensus sequence generator.

Thus far, I've been using Geneious's built in consensus sequence generator, which has worked well. The only issue is that it is not command line accessible.

I found that the samtools mpileup command shown at http://samtools.sourceforge.net/mpileup.shtml generates a consensus sequence of the same length as the Geneious consensus but is only ~93% identical.

What might be causing these differences? Does anyone else know of a good standalone consensus sequence generator?

Thanks


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