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adapter trimming agbt tech annotation assembly atac-seq bioanalyzer blast bowtie bowtie2 breakdancer bwa bwa sampe chip-seq cluster density cnvnator coverage cuffcompare cuffdiff cufflinks cuffmerge cummerbund de novo assembly deseq dexseq dindel edger error fastq fastqc fpkm gatk htseq-count illumina insert size ion torrent junction search failed logo metagenomics mirna miseq mpileup multiplexing newbler nextera ngs novoalign pacbio paired-end pindel prep_reads quality rna-seq rnaseq rpkm samtools samtools mpileup sanger/illumina 1.9 segmentation fault snp soap solid strand-specific tophat tophat2 tophat segment junction truseq varscan vcf vcftools velvet


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