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adapter trimming alignment amplicon sequencing annotation annovar assembly atac-seq bam bbmap bioanalyzer bioconductor bioinformatics blast bowtie bwa chip-seq cluster density covaris coverage cuffdiff cufflinks cuffmerge cummerbund de novo assembly deseq deseq2 dexseq differential expression edger error fastq fastqc fpkm gatk genome htseq-count illumina insert size ion torrent junction search failed metagenomics mirna miseq newbler nextera nextseq ngs normalization pacbio pindel quality rna-seq rnaseq rpkm sam samtools sanger/illumina 1.9 segmentation fault sequencing snp soap tophat tophat2 trimmomatic trinity truseq varscan vcf vcftools velvet


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