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Forum: Illumina/Solexa 02-26-2015, 01:01 PM
Replies: 5
Views: 2,243
Thanks Tom for your reply. It is very useful to...

Thanks Tom for your reply. It is very useful to know this.
Forum: Illumina/Solexa 02-24-2015, 06:56 AM
Replies: 5
Views: 2,243
I asked this question "Life Tech does not like...

I asked this question "Life Tech does not like Ampliseq on the Miseq" because the previous person commented that. So, do you think it is not an issue? Can we actually use Ampliseq for target...
Forum: Literature Watch 02-24-2015, 06:42 AM
Replies: 41
Views: 27,762
Thanks etal for your reply and this information....

Thanks etal for your reply and this information. I will look into OncoCNV.
Forum: Core Facilities 02-23-2015, 01:14 PM
Replies: 1
Views: 3,918
You can try Gentegra products.

You can try Gentegra products.
Forum: Literature Watch 02-23-2015, 09:57 AM
Replies: 41
Views: 27,762
Hi etal, I have a more basic question for CNV...

Hi etal, I have a more basic question for CNV detection- If I have a custom target panel through Ampliseq multiplex PCR, then is there a way to detect CNVs in that data?

Your CNVkit software works...
Forum: Sample Prep / Library Generation 02-23-2015, 09:41 AM
Replies: 15
Views: 3,875
"We suspect that KAPA quantification kit is not...

"We suspect that KAPA quantification kit is not optimal for quantifying this kind of libraries since the standards are longer than the library fragments."

You can do an adjustment of the length by...
Forum: Illumina/Solexa 02-23-2015, 08:02 AM
Replies: 5
Views: 2,243
I would also like to know more about why Life...

I would also like to know more about why Life Tech does not like Ampliseq on the Miseq?
Forum: RNA Sequencing 09-09-2014, 12:34 PM
Replies: 49
Views: 32,886
I will have to ask my bioinformatician for the...

I will have to ask my bioinformatician for the stats after adapter trimming. I will get back on this. Thanks for your time.
Forum: RNA Sequencing 09-09-2014, 11:57 AM
Replies: 49
Views: 32,886
Ok. I have looked at the FastQC report of the...

Ok. I have looked at the FastQC report of the samples and it looks fine to me. What kind of information should I give from FastQC report?
Forum: RNA Sequencing 09-08-2014, 01:05 PM
Replies: 49
Views: 32,886
The bioinformatic person is doing all that. She...

The bioinformatic person is doing all that. She trimmed the adapters and tried aligning the reads to the genome using tophat. She got 40% alignment there. We tried blasting some of the unaligned...
Forum: RNA Sequencing 09-08-2014, 12:45 PM
Replies: 49
Views: 32,886
Hi, I have run RNA-seq on human samples and got...

Hi, I have run RNA-seq on human samples and got very low alignment percentages in Tophat and RSEM. I had used Illumina ribo zero Truseq kit for library prep. What could be the reason of low...
Forum: Vendor Forum 08-01-2014, 12:35 PM
Replies: 7
Views: 5,384
Hi, I recently heard that Ribo Gold introduces a...

Hi, I recently heard that Ribo Gold introduces a bias while removing the rRNA, is it true? I was suggested not to use Illumina Truseq stranded total RNA protocol because of this.
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